RNAseq training by Harvard Chan Bioinformatics Core (HBC) - https://hbctraining.github.io/Training-modules/planning_successful_rnaseq/
https://github.com/zhxiaokang/RASflow/blob/master/Tutorial.pdf
https://bioconductor.org/packages/release/workflows/vignettes/rnaseqGene/inst/doc/rnaseqGene.html
complete step-by-step with scripts - https://bitbucket.org/adinasarapu/ibs_class/src/master/
Snakemake workflow - https://github.com/zhxiaokang/RASflow/blob/master/Tutorial.pdf
RNA-seq workflow: gene-level exploratory analysis and differential expression - https://bioconductor.org/packages/release/workflows/vignettes/rnaseqGene/inst/doc/rnaseqGene.html
Nanopore data analysis(NB: site hosts several protocols) - https://www.protocols.io/view/nanopore-data-analysis-14egn8xqpg5d/v2
RNAseq end-to-end analysis in R (RSubread) - https://www.alzheimersworkbench.ucsd.edu/EndToEndAnalysis_RNASeq.html
RNAseq end-to-end analysis (Good bash commands)
github.com/grimmlab/BookChapter-RNA-Seq-Analyses
scRNAseq - https://carmonalab.github.io/ProjecTILs_CaseStudies/Bassez_BC.html
scRNAseq - https://satijalab.org/seurat/articles/pbmc3k_tutorial.html
scRNAseq - https://carmonalab.github.io/ProjecTILs.demo/tutorial.html
scRNAseq - https://carmonalab.github.io/ProjecTILs_CaseStudies/Sandu_LCMV.htm
Workflow for a set of DEG - https://cytoscape.org/cytoscape-tutorials/protocols/differentially-expressed-genes/#/
RNA-seq Data Network Analysis ( with Cytoscape) - https://cytoscape.org/cytoscape-tutorials/protocols/rna-seq-data-analysis/#/
General RNA-seq workflow
Quality Control
2. Mapping
3. Process mapped results
4. Extract count table
5. Test for DEG
6. functional analysis
https://maayanlab.cloud/biojupies/help biojupies
Very good book on the underlying algorithms for most bioinformatics tasks. http://readiab.org/introduction.html
statOmics - https://gtpb.github.io/PDA19/
Elias JE, Gygi SP. Target-decoy search strategy for increased confidence in large-scale protein identifications by mass spectrometry. Nat Methods. 2007; 4:207–214.
Sticker, A., Martens, L. and Clement, L. (2017). Mass spectrometrists should search for all peptides, but assess only the ones they care about. Nature Methods 14, 643–644.
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